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In one study, intact GLP-1 [(736)NH 2 ] (measured using the sandwich ELISA from Linco Research, St Charles, MO, USA) concentrations of approximately 30 pmol/l peaked around 15 min after oral stimulation [31], strongly contrasting to the levels obtained by others [32,33,34,35] using sandwich ELISA techniques (which usually measure concentrations around 0 pmol/l), raising doubts as to the validity of this measurement

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