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The prevalence of any cigarette and/or smokeless tobacco use differed significantly by race/ethnicity in 21 states

RNA extraction, quantification of miRNA expression and qPCR Total RNA was extracted from the lung tissue samples of the screening cohort and from PBECs derived from non-COPD and COPD patients by TRIzol reagent method (Molecular Research Center, Cincinnati, OH) according to manufacturer's guidelines

It was the money. Silver lives across the river in Jeffersonville, Indiana, where the tax on cigarettes is 99 cents per pack