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To view the expression of key regulators across the trajectory on a shared scale, we normalized all smoothed expression values to be between zero and one, and then plotted these normalized expression curves across pseudotime (Fig

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For newly identified HBsAg-positive and CHB patients who have received antiviral therapy, HBV DNA should be detected using highly sensitive real-time quantitative PCR to identify patients with a low viral load, enabling the initiation of antiviral therapy as soon as possible or the timely adjustment of the treatment regimen