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Studies suggest that topical use reduces fine lines, wrinkles, and hyperpigmentation while protecting skin cells from UV-induced oxidative stress

In contrast, intraperitoneal hypertonic saline causes a rapid 8.6-fold increase in CRH hnRNA and mRNA within 15 minutes, returning to basal levels by 1 hour

CRH immunoreactivity is mainly found in the paraventricular nuclei (PVN) of the hypothalamus, often co-localized with AVP (51)

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Cells were maintained under standard conditions: cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin, and incubated at 37 C in a humidified environment with 5% CO